Table 3.
Medium | Factors | Quantity of somatic embryos (number per mL) | ||||
---|---|---|---|---|---|---|
number | ABA (mg/L) | PEG (g/L) | AC (g/L) | Maltose (g/L) | ||
>1 | 1 | 110 | 0.5 | 20 | 3.2 | |
2 | 1 | 130 | 1.0 | 25 | 2.4 | |
3 | 1 | 150 | 1.5 | 30 | 0.4 | |
4 | 2 | 110 | 0.5 | 30 | 8.4 | |
5 | 2 | 130 | 1.0 | 20 | 34.9 | |
6 | 2 | 150 | 1.5 | 25 | 14.9 | |
7 | 3 | 110 | 0.5 | 25 | 14.6 | |
8 | 3 | 130 | 1.0 | 30 | 9.5 | |
9 | 3 | 150 | 1.5 | 20 | 24.4 | |
yj1 | 6 | 26.2 | 27.6 | 62.5 | ||
yj2 | 58.2 | 46.8 | 35.2 | 31.9 | ||
yj3 | 48.5 | 39.7 | 49.9 | 18.3 | ||
2 | 8.7 | 9.2 | 20.8 | |||
19.4 | 15.6 | 11.7 | 10.6 | |||
16.2 | 13.2 | 16.6 | 6.1 | |||
Rj | 17.4 | 6.9 | 7.4 | 14.7 | ||
Primary and secondary factor | ABA>Maltose>AC>PEG | |||||
Optimal composition | ABA2 + PEG2 + AC3 + Maltose1 |
ABA, abscisic acid; PEG, polyethylene glycol 8000; AC, activated carbon. yjk (k=1, 2, 3) is the sum of test results with the same level of k in the jth column of the table; is the mean value of the test results with the same level of k in the jth column of the table; Rj is the range of .Rj=max( )- min( ).