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. 2000 Nov;74(21):9980–9986. doi: 10.1128/jvi.74.21.9980-9986.2000

FIG. 1.

FIG. 1

Schematic diagrams of plasmid constructs. (A) pTZVT416 containing a permuted full genome of VT416 (nt 2762 to 3852 and nt 1 to 2761), carrying a putative promoter (black area), TATA box, and the poly(A) signal (open circle) in the sense orientation [polyA(S)]; (B) pBK-CMV containing the SV40 replication origin (SVo), SV40 poly(A) signal (SVpA), lacZ, multiple cloning sites (MCS), and CMV promoter (CMV); (C) pBK* lacking the CMV promoter from pBK-CMV; (D) pBK*VT416(1.3G) containing 1.3 genome lengths of VT416 (nt 2762 to 3852 and nt 1 to 3770) (terminal SmaI site in parentheses because of its interruption in the construct); (E) pBK*VT416(1.5G) containing 1.5 genome lengths of VT416 (nt 2762 to 3852 nt 1 to 3852 and nt 1 to 971) with a putative promoter region and a poly(A) site in the antisense orientation [polyA(aS)]. The predicted orientations of mRNAs are shown by curved arrows in the peripheries of panels D and E. Restriction sites are shown for EcoRI (E), PstI (P), SmaI (S), and AseI (A).