FIG. 7.

ICP0 induces the accumulation of conjugated ubiquitin at mitotic centromeres. (A and B) A mitotic cell infected with vEG110 and stained with MAb FK2. (C and D) A mitotic cell infected with HSV-1 strain 17+ costained with MAb FK1 and r191 to detect ICP0. (E and F) Corresponding uninfected controls. (G and H) HEp-2 cells singly transfected with pCIQEUb52 and later infected with HSV-1 strain 17+. This view shows a rare transfected cell that was infected in the late G₂ phase of the cell cycle and has arrested in mitosis with the ICP0-induced localization of tagged ubiquitin at centromeres.