TABLE 1.

Plasmids used in this study

Name	Mutationf	Activationa	Repressionb	Increased FK2 detection of conjugated ubiquitinc
pCI110	Wild type	+++	NAd	+++
pCIFXE	del 106–149	−	−	−
p110DR40	del 129–130	−	NDe	−
p110K144E	Substitution	−	ND	−
p110W146A	Substitution	+++	ND	+++
p110Q148E	Substitution	++	ND	+
p110N151D	Substitution	+	ND	−
p110F1	ins 150	−	−	−
p110D22	del 162–188	−	−	−
p110R2	ins 162	−	−	−
p110E8	ins 188	−	−	−
p110E13	ins 197	−	−	−
p110R3	ins 212	−	−	−
p110E32-1	ins 222	−	−	+
p110D13/32	del 197–222	ND	ND	−
p110262	1–241	ND	+	++
pCIM1	R623L, K624I	−	NA	+++
p110D12	del 594–633	+	NA	+++
p110E52X	1–593	++	NA	+++

^aData taken from references 8, 16, 21, and 42. The effects of the mutations on the activation of a target expression cassette by ICP0 alone were estimated, using slightly different conditions in the three series of experiments; −, less than 10% of wild-type activity; +, 10 to 40%; ++, 40 to 70%; +++, >70%. 

^bTruncated forms of ICP0 containing the RING finger region repress expression from reporter constructs (50). p110262 is a plasmid equivalent to the repression construct used in those studies; + and − indicate the effects of these same mutations in this region in their assay. 

^cAbility of plasmid construct to induce increased conjugated ubiquitin staining detected by MAb FK2 in transfected cells. 

^dNA, not applicable. 

^eND, not done. 

^fins, insertion; del, deletion.