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Effect of ICP0 USP7 binding mutants on the SUMO-1 conjugation of PML. HEp-2 cells were cotransfected with pPML(F) and the plasmids as shown, using Lipofectamine PLUS. Samples harvested 24 h posttransfection were analyzed by Western blotting using MAb anti-F at a dilution of 1/5,000.
