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Expression of tagged ICP0 homologues. (A) HEp-2 cells were transfected with pUC9 (lane M) or plasmids expressing pp65-ICP0 or the pp65 homologues, as indicated. Samples were harvested into SDS-gel loading buffer at 24 h posttransfection and analyzed by Western blotting using MAb anti-pp65 at a dilution of 1/750. The positions of the molecular weight markers are indicated. (B) Longer exposure of the EHV and VZV tracts of the blot.
