FIG. 7.

Effect of 17Eg63 on cellular proteins. (A) HFL cells were mock infected or infected with wild-type 17syn+ virus (lane WT) or ICP0 mutants and recombinant as shown at 10 PFU per cell in the presence (+) or absence (−) of 5 μM MG132. Cells were harvested into SDS-gel loading buffer at 4 h postadsorption and analyzed by Western blotting. The blot was probed with anti-PML antibody E510 at 1/5 and reprobed with anti-CENP-C antibody r554 at 1/1,000 and anti-ICP4 MAb 10176 at 1/5,000. (B) A further blot was probed with polyclonal anti-Sp100 SpGH at 1/1,000 and reprobed with MAb anti-ICP4 10176 at 1/5,000. The positions of the PML and Sp100 isoforms and CENP-C are indicated, as are molecular weight markers. Long and short dashes indicate unmodified and modified proteins, respectively.