FIG. 9.

Effect of ICP0 on the SUMO-1 conjugation of PML in the presence of exogenous SUMO-1. Using Lipofectamine PLUS, HEp-2 cells were cotransfected with pPML(F) and either pUC9 or pp65-ICP0 (lanes D) or triple transfected with pPML(F), pCIPIC1, and either pUC9 or pp65-ICP0 (lanes T). Samples were harvested into SDS-gel loading buffer at 24 h posttransfection and analyzed by Western blotting using MAb anti-F at a dilution of 1/5,000. The positions of unmodified PML(F) and PML(F) modified by exogenous SUMO-1 in the triple transfections are indicated by long and short bars, respectively.