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. Author manuscript; available in PMC: 2023 May 23.
Published in final edited form as: Methods Mol Biol. 2023;2566:113–132. doi: 10.1007/978-1-0716-2675-7_9

Table 1.

Tissue Embedding Methods

Tissue Fixation method Embedding material
Skeletal muscle No fixation Cryopreservation in OCT
Skeletal muscle with endogenous fluorophore proteins Cardiac perfusion fixation with 4% PFA followed by immersion in 2% PFA overnight Sucrose gradient followed by cryopreservation in OCT
Whole murine embryos (E9.5 to E18.5) Immersion fixation in 4% PFA for 12–48 hour (age dependant) Paraffin embed or sucrose gradient followed by cryopreservation in OCT (cryopreservation provides lower resolution images of somite stage embryos)
Murine embryo limbs (E14.5 to E18.5) No fixation Cryopreservation in OCT
Murine embryo with endogenous fluorophore Immersion fixation in 4% PFA (see Note 22) Sucrose gradient and OCT cryopreservation