Skeletal muscle |
No fixation |
Cryopreservation in OCT |
Skeletal muscle with endogenous fluorophore proteins |
Cardiac perfusion fixation with 4% PFA followed by immersion in 2% PFA overnight |
Sucrose gradient followed by cryopreservation in OCT |
Whole murine embryos (E9.5 to E18.5) |
Immersion fixation in 4% PFA for 12–48 hour (age dependant) |
Paraffin embed or sucrose gradient followed by cryopreservation in OCT (cryopreservation provides lower resolution images of somite stage embryos) |
Murine embryo limbs (E14.5 to E18.5) |
No fixation |
Cryopreservation in OCT |
Murine embryo with endogenous fluorophore |
Immersion fixation in 4% PFA (see
Note 22) |
Sucrose gradient and OCT cryopreservation |