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. Author manuscript; available in PMC: 2023 May 23.
Published in final edited form as: Int J Pharm. 2020 Nov 27;593:120119. doi: 10.1016/j.ijpharm.2020.120119

Figure 4.

Figure 4.

Overview of the separation process using AF488 OVA and M-A-SNPs. A magnet was used to separate the supernatant (Sample 1), which should show any free MAs or antigen. Then, the M-A-SNPs were resuspended in WFI and transferred to a clean vial to separate them from any of the free-form components. This was accomplished using several washes while recycling the water wash (Sample 2). MAs were then extracted from the separated beads obtained after the previous steps using Triton X-100 several times and the extracts were collected and analyzed undiluted (Sample 3).