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Activation of Mincle in response to MAs-coated A-SNPs. HEK cells transfected with (a) mouse Mincle, or (b) human Mincle. An NF-KB-driven SEAP reporter were added to the indicated formulations in 96 well plates and incubated with the compounds for 18–24 h followed by assessment of the supernatants for SEAP levels. Data are represented as fold change in OD650 over vehicle treated cells. Three replicates (from three independent donors) mean +/− SEM.
