FIG. 2.

Indirect immunofluorescence of stable VP22-expressing (V49 and 14349) and control neomycin-resistant (V202 and 14320) cell lines doubly labeled with antibodies specific for VP22 and α-tubulin. Cells were fixed, permeabilized, and stained with affinity-purified RGST49 and monoclonal antibody to α-tubulin as described in Materials and Methods. (A, D, G, and J) VP22 staining; (B, E, H, and K) α-tubulin staining; (C, F, I, and L) merged images. A larger view of the area designated by the dotted boxes in A, B, and C is shown in the inset at the lower right of each image.