FIG. 6.

HSV-1(F) and RF177 single-step growth curves in Vero cells. Confluent monolayers of Vero cells were infected at an MOI of 5.0 PFU per cell as described in Materials and Methods. At 0, 2, 4, 6, 8, 10, 12, and 24 h p.i., stocks were prepared and counted in duplicate on Vero cells. Each point represents the average of three independent experiments. HSV-1(F) growth is indicated by a solid line and solid diamonds, and RF177 growth curve is shown as a dotted line and open squares.