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. 1981 Jul;36(7):512–516. doi: 10.1136/thx.36.7.512

Serum and sputum alpha 2 macroglobulin in patients with chronic obstructive airways disease.

D Burnett, R A Stockley
PMCID: PMC1020433  PMID: 6171904

Abstract

Serum alpha 2 macroglobulin concentrations were measured in patients with chronic obstructive airways disease and an age-matched group of control subjects. The mean serum level of alpha 2 macroglobulin was significantly lower in bronchitic subjects with acute chest infections than the mean value of the controls. No significant differences were observed between serum alpha 2 macroglobulin values in controls, subjects with "emphysema", and bronchitic patients who did not have chest infections. Sputum alpha 2 macroglobulin concentrations were compared in sputum samples from bronchitic patients with and without acute chest infections. The protein was detected (greater than 0.2 mg/l) in 94% of sputum samples from infected subjects but only 60% of non-infected sputum samples. Concentrations of alpha 2 macroglobulin in infected samples were significantly higher than the non-infected samples. Sputum/serum concentration ratios of alpha 2 macroglobulin were also significantly higher in infected samples but this difference was eliminated by "correcting" the values with the albumin sputum/serum ratios of the same samples. The results suggest that alpha 2 macroglobulin concentrations are higher in bronchial secretions during chest infection because of increased transudation from the blood rather than the presence of significant local secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnett T. B., Gottovi D., Johnson A. M. Protease inhibitors in chronic obstructive pulmonary disease. Am Rev Respir Dis. 1975 May;111(5):587–593. doi: 10.1164/arrd.1975.111.5.587. [DOI] [PubMed] [Google Scholar]
  2. Barrett A. J., Brown M. A., Sayers C. A. The electrophoretically 'slow' and 'fast' forms of the alpha 2-macroglobulin molecule. Biochem J. 1979 Aug 1;181(2):401–418. doi: 10.1042/bj1810401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnett D., Stockley R. A. The electrophoretic mobility of alpha 1-antitrypsin in sputum and its relationship to protease inhibitory capacity, leucocyte elastase concentrations and acute respiratory infection. Hoppe Seylers Z Physiol Chem. 1980 May;361(5):781–789. doi: 10.1515/bchm2.1980.361.1.781. [DOI] [PubMed] [Google Scholar]
  4. Debanne M. T., Bell R., Dolovich J. Characteristics of the macrophage uptake of proteinase-alpha-macroglobulin complexes. Biochim Biophys Acta. 1976 Apr 23;428(2):466–475. doi: 10.1016/0304-4165(76)90055-6. [DOI] [PubMed] [Google Scholar]
  5. Erikson S. Proteases and protease inhibitors in chronic obstructive lung disease. Acta Med Scand. 1978;203(6):449–455. [PubMed] [Google Scholar]
  6. Eriksson S. Studies in alpha 1-antitrypsin deficiency. Acta Med Scand Suppl. 1965;432:1–85. [PubMed] [Google Scholar]
  7. Galdston M., Levytska V., Liener I. E., Twumasi D. Y. Degradation of tropoelastin and elastin substrates by human neutrophil elastase, free and bound to alpha2-macroglobulin in serum of the M and Z (Pi) phenotypes for alpha1-antitrypsin. Am Rev Respir Dis. 1979 Mar;119(3):435–441. doi: 10.1164/arrd.1979.119.3.435. [DOI] [PubMed] [Google Scholar]
  8. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  9. Ohlsson K., Laurell C. B. The disappearance of enzyme-inhibitor complexes from the circulation of man. Clin Sci Mol Med. 1976 Jul;51(1):87–92. doi: 10.1042/cs0510087. [DOI] [PubMed] [Google Scholar]
  10. Ohlsson K., Tegner H. Granulocyte collagenase, elastase and plasma protease inhibitors in purulent sputum. Eur J Clin Invest. 1975 Jun 12;5(3):221–227. doi: 10.1111/j.1365-2362.1975.tb00448.x. [DOI] [PubMed] [Google Scholar]
  11. SIMON G. RADIOLOGY AND EMPHYSEMA. Clin Radiol. 1964 Oct;15:293–306. doi: 10.1016/s0009-9260(64)80001-5. [DOI] [PubMed] [Google Scholar]
  12. Stockley R. A., Burnett D. Alpha,-antitrypsin and leukocyte elastase in infected and noninfected sputum. Am Rev Respir Dis. 1979 Nov;120(5):1081–1086. doi: 10.1164/arrd.1979.120.5.1081. [DOI] [PubMed] [Google Scholar]
  13. Stockley R. A., Burnett D. Serum derived protease inhibitors and leucocyte elastase in sputum and the effect of infection. Bull Eur Physiopathol Respir. 1980;16 (Suppl):261–272. doi: 10.1016/b978-0-08-027379-2.50027-2. [DOI] [PubMed] [Google Scholar]
  14. Stockley R. A., Mistry M., Bradwell A. R., Burnett D. A study of plasma proteins in the sol phase of sputum from patients with chronic bronchitis. Thorax. 1979 Dec;34(6):777–782. doi: 10.1136/thx.34.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tegner H. Quantitation of human granulocyte protease inhibitors in non-purulent bronchial lavage fluids. Acta Otolaryngol. 1978 Mar-Apr;85(3-4):282–289. doi: 10.3109/00016487809111936. [DOI] [PubMed] [Google Scholar]

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