TABLE 4.

Coentry of HCV and α-sarcin increases with higher levels of LDLr expression^a

Infection conditions	Protein synthesis (cpm)b in:
	MOLT-4 (log)	MOLT-4 (stationary)	MOLT-4 LPDS
Mock (1.08 g/ml)	24,541	21,600	22,000
HCV (1.08 g/ml)	19,877	3,020c	3,800c
No α-sarcin	22,371	23,428	21,670

^aCells were incubated with low-density HCV or Mock preparations (1.08 g/ml) in the presence or absence of α-sarcin as described in Materials and Methods. MOLT-4 (log) cells were in the logarithmic phase and demonstrated low levels of LDLr expression. Stationary MOLT-4 cells and MOLT-4 cells grown in LPDS expressed significantly higher levels of LDL receptor (see Fig. 2). Cellular protein synthesis was determined 1 h postincubation by labeling with [³⁵S]methionine as described in Materials and Methods. 

^bData represent [³⁵S]methionine incorporation. 

^cHCV cpm values in the MOLT-4 stationary and LPDS cells were significantly different from Mock, no α-sarcin, and HCV binding to (log) stage MOLT-4 cells (P < 0.01; paired t test).