EM of RK13 cells infected with wild-type PrV. Cells were embedded in Lowicryl K4M and reacted with monospecific antiserum against the UL34 protein (A, C, E, and G) or the UL49 protein (B, D, F, and H). (A and B) Mature extracellular virions. (C and D) Intracytoplasmic enveloped virions. (E and F) Nuclear membrane. (G and H) perinuclear virions. C, cytoplasm; N, nucleus. Bar, 250 nm.