FIG. 2.
Spinoculation enhances HIV-1 viral adsorption to CEM-SS cells and RT to similar extents. The numbers of copies of viral RNA and cDNA (extended first strand) per cell were measured immediately after challenge of 2 × 105 cells with 100 μl of a 3.0-μg/ml p24gag stock of HIV-1IIIB at 1 × g or 1,200 × g using kinetic PCR. The numbers of copies of viral cDNA per cell were also measured after 5 h in culture. At 24 h, total levels of p24gag (cell associated and supernatant) were determined by ELISA and converted to the number of viral particles produced per cell. UD, undetectable.