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The modest g forces used for spinoculation sediment HIV-1 particles. One-hundred-microliter aliquots of an HIV-1 viral stock were exposed to 1 × g or 1,200 × g for 2 h in a 96-well tissue culture microtiter plate at room temperature in the absence of cells. Serial 20-μl aliquots were then withdrawn from the upper meniscus and assayed for the presence of p24gag by ELISA. The data shown represent the means of three independent sample sets.
