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. 2023 May 19;18:2677–2691. doi: 10.2147/IJN.S398046

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© 2023 Zhu et al.

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Proliferation and apoptosis of FA-Res liposome-treated osteosarcoma cells and their associated signaling pathways. (A) Cellular uptake of free COU-6, COU-6 liposomes, and FA-COU-6 liposomes by 143B cells. (B) Cell viability of 143B cells after 48 h treatment with free Res, Res liposomes, and FA-Res liposomes. (C and D) The clonogenic capacity of 143B cells and their colony number were calculated. (E and F) Apoptosis analysis of 143B cells by double staining with FITC Annexin V/PI and the percentage of apoptosis was calculated. (G) Expression of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3, and cytochrome C proteins in 143B cells was detected by western blot analysis. All data are expressed as mean ± SD (n=3). *p<0.05, **p<0.01 and ***p<0.001.