FIGURE 4.

Interleukin-1 receptor-associated kinase (IRAK)-1 expression is reduced in COPD bronchial epithelial cells. a) Total protein isolated from normal and COPD mucociliary-differentiated cultures was subjected to electrophoresis, proteins transferred to nitrocellulose membranes, blocked with 5% bovine serum albumin, and subjected to Western blot analysis with myeloid differentiation primary response gene 88 (MyD88), IRAK-4, IRAK-1 or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. b), c) and d) The intensity of the bands was quantified by ImageJ software and levels of MyD88, IRAK-4 and IRAK-1 were expressed as fold change over GAPDH. Data represent mean±sd calculated from 4–6 normal and COPD cultures, and statistical significance calculated by unpaired t-test.