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. 2023 May 18;83(10):1573–1587.e8. doi: 10.1016/j.molcel.2023.04.015

Figure 2.

Figure 2

Degradation of topoisomerases results in refractory periods

(A) Schematic depicting the homozygous tagging of diploid yeast (2n) of endogenous TOP1 and TOP2 with an auxin-inducible degron, and homozygous OsTIR1 insertion at the his3Δ1 locus.

(B and C) MS2-PP7 cross-correlation of the GAL1-GAL10 in cells without (−IAA, left, n = 214 cells [cis], n = 126 cells [trans]) and with auxin (+IAA, right, n = 158 cells [cis], n = 112 cells [trans]) for the cis (dark blue) and trans (orange). Arrows indicate example valleys indicating refractory periods. Shaded area indicates SEM.

(D and E) Same as (B) and (C) for GAL10-GAL7. −IAA: n = 118 cells (cis), 102 cells (trans), +IAA: n = 143 cells (cis), n = 128 cells (trans).

See also Figures S1 and S2.