Mice were treated with either cHBI (20 nmol) or PBS post sensitization with a cocktail of six Ara h 2 specific IgE (1 μg) and then challenged intravenously with Ara h 2 after 24 hours, 72 hours, 7 days or 14 days. At 24, 72, 168 hour time points the Ara h 2 dose was 1ng; on day 14 the dose was 10 ng. (A-D) Change in core body temperature at the indicated time points post cHBI treatment. (E) Maximal change in body temperature post Ara h 2 challenge in the PBS-treated mice and cHBI-treated mice. Data represent mean ±SEM, (n = 4). Data are representative of two independent experiments. (F) Scatter plot of serum tryptase levels from mice at the indicated time points (n = 4). Data are representative of two independent experiments. One way ANOVA with Tukey post hoc test was used to compare means in (A-D). An unpaired Mann Whitney test was used to compare means in (F). ***P <0.001, **P <0.01, *P <0.05. Dashed line in F indicates different allergen dose.