Fig. 8: cHBI inhibited anaphylaxis and mast cell activation to oral Ara h 2 allergen challenge.

(A) Change in core body temperature in PBS-treated mice and cHBI (20 nmol) treated mice post Ara h 2 allergen (25 μg) oral challenge (B) Kaplan-Meyer survival plot for PBS- and cHBI-treated mice post oral Ara h 2 allergen challenge. Data represent mean ±SEM pooled from two independent experiments (n = 12). (C) Maximal change in body temperature post oral Ara h 2 allergen challenge in PBS-treated mice and cHBI-treated mice. Data represent mean ±SEM, (n = 12) (D) Scatter plot of serum tryptase levels in PBS- and cHBI-treated mice (n = 12). Data are pooled from two independent experiments. (E) Representative flow plot showing surface expression levels of degranulation markers CD107a, and CD63 on peritoneal mast cells. (F) Scatter plots of the percentage of CD107a, and CD63 positive cells (n = 6–7). Data are representative of two independent experiments. (G) Chemokine (CCL2, CCL3, and CXCL8) and cytokine (IL-6, and TNF-α) serum concentrations post Ara h 2 allergen oral challenge in PBS-treated mice and cHBI-treated mice (n = 4). Data are representative of two independent experiments. (H) Change in core body temperature in mice treated with PBS or cHBI two minutes after oral allergen challenge. Arrow indicated time of cHBI administration. (I) Kaplan-Meyer survival plot for mice treated with PBS or cHBI two minutes after oral allergen challenge. (J) Scatter plot of serum tryptase levels in mice treated with PBS or cHBI two minutes after oral allergen challenge. Data represent mean ±SEM. Data are pooled from two independent experiments (n=10). One way ANOVA with Tukey post hoc test was used to compare means in (A), and (H). A log-rank (Mantel-Cox) test was used for statistical analysis in (B), and (I). An unpaired Mann Whitney U test was used to compare means in (D), (F), (G), and (J). ***P <0.001, **P <0.01, *P <0.05.