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. 2023 Jan 12;164(3):bqad008. doi: 10.1210/endocr/bqad008

Figure 4.

Figure 4.

Refeeding stimulates tropomyosin receptor kinase B (TrkB) activation that is dependent on regulated brain-derived neurotrophic factor (BDNF) release. A and B, Immunoblotting analysis and quantification of TrkB activation in hypothalami of 10-week-old female wild-type (WT) mice that were fasted overnight and then refed either chow or HFD for 1 hour. One-way analysis of variance (ANOVA) with Tukey multiple comparison test: **P less than .01, ***P less than .001, and ****P less than .0001. C and D, Immunoblotting analysis and quantification of TrkB activation in hypothalami of 10-week-old female WT mice that were fasted overnight and then refed a high-fat diet (HFD) for 1 hour. **P less than .01 by unpaired t test. E and F, Immunoblotting analysis and quantification of TrkB activation in hypothalami of 10-week-old female BdnfMet/Met mice that were fasted overnight and then refed HFD for 1 hour. ns, no significance by unpaired t test. All data are shown as mean ± SEM.