FIG. 1.

ICP0 protein accumulates in the nuclei of nonneuronal cells in dissociated TG cultures. Sixteen hours after infection with TOZ (MOI of 5), ICP0 is found in the nucleus of Schwann cells (arrowheads) but not neurons (arrow, identified by the characteristic appearance) in the cultures (phase contrast [A], fluorescence [B], and merged image [C]). At higher power, the immunostaining in the nucleus of the nonneuronal cells has the characteristic punctate appearance as described previously (D). ICP0 RNA can be detected in infected neurons by in situ hybridization with a digoxigenin-labeled riboprobe (inset, panel A).