FIG. 2.

ICP0 does not accumulate in the nucleus of neurons in culture. The identity of the neurons was confirmed by immunostaining with an antibody to the neuron-specific antigen MAP2 (C and D). TOZ (A and C)- and mock (B and D)-infected cultures, 16 h postinfection, show ICP0 in a punctate pattern in the nucleus of Schwann cells but not of neurons in the infected cultures (A and C). X-Gal staining (inset, panel C) demonstrates that the majority of the cells in the culture, including neurons, were infected and expressed the lacZ transgene. Panels A and B are fused phase and fluorescent images of cultures counterstained with DAPI (4′,6′-diamidino-2-phenylindole).