FIG. 2.

Agarose gel electrophoresis of amplicons specific to the positive (A) and negative (B) strands of EV genomes. Strand-specific RT-PCR was carried out on total RNA taken from mock-infected islets (lane 2) and CVB4 E2-infected islets at 4 h, 7 days, 15 days, and 30 days p.i. (lanes 3 to 6, respectively). Lane 1 corresponds to a 100-bp DNA molecular size ladder. Equivalent expression of β-actin mRNA was detected in all samples (C). Similar results were obtained with CVB3 and CVB4 JBV and with islets from eight adult brain-dead donors.