FIG. 1.

Diagrams representing the pRPVINS-HA and pRPVANC-GFP antigenome cDNA constructs, made as described in Materials and Methods. (A) The pRPVINS-HA construct was made by inserting the mutant HA ORF into the unique AscI cloning site in the gene expression cassette, located between the P and M genes of pRPVINS. (B) The pRPVANC-GFP construct was made by inserting the ANC-GFP ORF into the unique AscI cloning site in the gene expression cassette, which includes an N-terminal secretory signal sequence (SIG) located between the P and M genes of pRPVSIG. ORFs of RPV: N, nucleocapsid; P, phosphoprotein; M, matrix; F, fusion; H, hemagglutinin; L, large protein of RPV. UTR, untranslated region represented in black in the complete genome diagram; i, intergenic triplet; T7, T7 RNA polymerase promoter; δ, hepatitis delta ribozyme; ττ, T7 RNA polymerase terminators.