FIG. 4.

Cell surface expression and localization of HA, ANC-GFP, and RPV H in recombinant RPV-infected cells using GFP fluorescence and indirect immunofluorescence confocal microscopy. Shown are transmitted light (A to E) and immunofluorescence (F to J) images of RPVII-infected B95a cells, transmitted light (K and L) and immunofluorescence (P and Q) images of RPVINS-HA-infected B95a cells, and transmitted light (M to O), immunofluorescence (R and S), and GFP fluorescence (T) images of RPVANC-GFP-infected B95a cells. Virus-infected B95a cells were fixed at 2 days postinfection, and nonpermeabilized cells were used for surface protein labeling as described in Materials and Methods. The relevant virus is indicated at the top, and antibody specificity is indicated on the left. Anti-RPV-H (αRPV-H), anti-HA (αHA), and anti-GFP (αGFP) antibody immunofluorescence is red; GFP fluorescence (GFP) is green. Original magnification of photomicrographs was ×1,000.