Fig. 4.

TurboID does not impact cellular phenotypes.A, differential expression analysis (DEA) comparing whole cell (WC) transduced BV2 cell lysates with untransduced BV2 cell lysates identifies 53 differentially expressed proteins (DEPs) in BV2 with TurboID expression. B, DEA comparing WC transduced and untransduced N2A identifies 74 DEPs. C, principal component analysis (PCA) of cytokine profiles derived from cultured supernatants indicates PC1 captures 94% of the variance across samples, accounting for differences in cell type. PC2 captures 4% of the variance, accounting for lipopolysaccharide (LPS) impact on BV2 cytokines. D, linear regression of LPS cytokine fold-change induction of untransduced (x-axis) and transduced (y-axis) BV2 cells demonstrates high correlation (R² = 0.83). E, bar graph depicting basal, ATP-linked, and maximal cellular respiration rates of transduced and untransduced BV2 cells. LPS significantly decreases the maximal respiration of untransduced BV2 cells (p = 0.0004) and decreases maximal respiration in transduced BV2 cells, though this finding is statistically insignificant (p = 0.13). F, oxygen consumption rate (OCR) traces for transduced and LPS exposed BV2 cells highlight LPS response in maximal respiration. G, extracellular acidification rate (ECAR) highlights the basal glycolytic rate as higher when cells are exposed to LPS but are not impacted by transduction status.