Table 1.
Antibodies used and their corresponding dilutions
| Antibody | Manufacturer | Catalog number | Dilution |
|---|---|---|---|
| Rabbit anti-V5 | Abcam | ab206566 | 1:500 |
| Rat anti-α-tubulin | MilliporeSigma | MAB1864 | 1:1000 |
| Goat anti-β-actin | Santa Cruz Biotechnology | sc-1615 | 1:1000 |
| Rabbit anti-histone H3 | Abcam | ab1791 | 1:5000 |
| Donkey anti-rabbit 800 | Invitrogen | A11374 | 1:10,000 |
| Donkey anti-goat 680 | LI-COR | A21084 | 1:10,000 |
| Donkey anti-goat 800 | LI-COR | A11370 | 1:10,000 |
| Goat anti-rat 800 | LI-COR | 926-32219 | 1:10,000 |
| Streptavidin DyLight 594 | ThermoFisher | 21842 | 1:1000 |
| Streptavidin, Alexa-Fluor 680 conjugate | Invitrogen | S32358 | 1:10,000 |
| DAPI | Roche | 10236276001 | 1 μg/ml |