Table 2.
Reagents used and their manufacturer and catalog numbers
| Reagent | Manufacturer | Catalog number |
|---|---|---|
| StartingBlockT20 | ThermoFisher | 37543 |
| HALT protease & phosphatase inhibitor cocktail | ThermoFisher | 78446 |
| Dulbecco’s modified Eagle's medium | Gibco | 11965-092 |
| Penicillin–streptomycin | Gibco | 15140-122 |
| Fetal bovine serum (FBS) | Gibco | 26140-7079 |
| Biotin | Sigma–Aldrich | B4639-100mg |
| LPS | Sigma–Aldrich | L4391-1mg |
| Puromycin | Sigma–Aldrich | P9620 |
| 4% Paraformaldehyde in PBS | Thermo Scientific | J19943-K2 |
| 0.05% Trypsin–EDTA | Gibco | 253000054 |
| Reagent A | ThermoFisher | 23222 |
| Reagent B | ThermoFisher | 23224 |
| Bovine serum albumin standards | ThermoFisher | 23208 |
| LB medium | Sigma–Aldrich | 28713-500G-F |
| Seahorse Flux Pack | Agilent | 102601-100 |
| Seahorse XF Media | Agilent | 103575-100 |
| Sodium pyruvate | Sigma–Aldrich | S8636-100mL |
| l-glutamine | Sigma–Aldrich | G7513-100mL |
| Glucose | Sigma–Aldrich | G8769-100mL |
| Oligomycin | Sigma–Aldrich | 75351-5mg |
| Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone | Sigma–Aldrich | C2920-10mg |
| Rotenone | Sigma–Aldrich | R8875-1G |
| Antimycin-A | Sigma–Aldrich | A8674-25mg |