FIG. 7.

Detection by RT-PCR of meq and antisense meq transcripts in QT35 cells, QT35 cells cocultivated with CEF (QT35/CEF), QT35 cells cocultivated with HVT-infected CEF (QT35/HVT), CEF infected with HVT (CEF/HVT), and CEV infected with CVI988 (CEF/CVI988). CEF/HVT and CEF/CVI988 were used as negative and positive controls, respectively. cDNA was prepared using reverse transcriptase (RT); a PCR assay in the absence of RT was used to determine if contaminating DNA was present. (A) Locations of the 5meq/meqsRT and 3meqRTanti primers in relation to the meq and antisense meq ORFs and ORF L1. The predicted amplicon is indicated by a vertically oriented box and will detect nonspliced meq mRNA or antisense ORF-1, depending on the primer used to generate the cDNA. (B) Detection of transcripts by RT-PCR using random-hexamer primers, 3meqRTanti, or 5meq/meqsRT to obtain cDNA. The sizes of the amplified fragments and the types of transcripts are indicated at the right. The PCR products were separated by electrophoresis in 1.5% agarose gels, stained with ethidium bromide, and visualized with an Eagle Eye II still-video system.