TABLE 1.

Primer pairs used for amplification of QMDV fragments from QT35 cells by PCR and RT-PCR

MDV gene	GenBank data		Primer sequences (5′ to 3′)	Size (bp)	Primer
	Accession no.	Nucleotides
ICP4	U17705	6978–6953	CTTTATGCGACTGGCGTGCTGCCGTA	674	ICP4-5′a
		6305–6328	TCCTCCTGCACGCCTGTTTGCGGT		ICP4-3°a
LAT	U17705	6242–6261	TGTCACCTGAATATCATTGC	329	LATs2-5
		6570–6551	CCTCTTCATCTTCCTCCTCT		LATs2-3
ICP22	L22174	1282–1302	ATGAGAGCGACGACGAAGATT	331	5′RT ICP22
		1612–1595	AGAGTCCTGCGCAGATGTGGA		3′RT ICP22
ICP27	U10040	2167–2188	CAAGAAGGGCATCACCGAAGAA	31	5′RT ICP27
		2482–2461	GACCCGCATGCAATAAATACTC		3′RT ICP27
meq	M89471	431–450	GGTCGACTTCGAGACGGAAA	337	5meq/meqsRT
		767–748	GTAAGCAGTCCAAGGGTCAC		3meqRTanti
pp38	M73484	1303–1320	CGAGGACGGCGAGAAATG	404	5′pp38
		1706–1686	TTAATGCGCGAACGGAATGTA		3′pp38
VP16	L10283	2069–2091	GGCCGATTTACCTTCTTATGTAG	451	5′VP16
		2519–2500	CCGGAGTTCAGGAGCAGTC		3′VP16
ORF L1	L19763	869–892	TTGTTCACTGTGCGGCATTATTAC	345	5′RT ORF L1
		1213–1192	TACCGGCACCGACAGTTCTTTA8		3′RT ORF L1
pp14	D13389	5804–5823	GAGGTTCTGGCAGAGATTCC	542	pp14F
		6345–6325	CTGCTTGTATGCTACAACGGC		pp14R
gB	D13713	1292–1315	GTGCAACAGTTAATGGGAGATACA	457	5′gB
		1748–1725	AGCATGGCGAATTGAACAGACGAT		3′gB

^aData are from Xing et al. (68).