TABLE 5.
MDV transcripts in QT35 cells in the presence and absence of CEF infected with HVT
| Cells (treatment) | MDV transcripts for a:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| ICP4b | ICP22 | ICP27 | VP16 | LATsc | ORF L1 | meq | pp38 | gB | |
| QT35 | + | − | − | − | + | + | + | − | − |
| QT35 (CEF) | + | − | − | − | + | + | + | − | − |
| QT35 (HVT) | ++ | + | + | + | + | + | + | + | + |
| CEF (HVT) | − | − | − | − | − | − | − | − | − |
| CEF (CVI988) | + | + | + | + | + | + | + | + | + |
a
++, increased transcription compared to QT35; +, transcripts present; −, no transcripts detected.
b
The strand-specific primer LATs2-3 was used to generate cDNA, and cDNAs were detected by Southern hybridization.
c
The strand-specific primer LATs2-5 was used to generate cDNA, and cDNAs were detected by Southern hybridization.