Figure 2.

Case summary: baseline infection (case A2). Key events and laboratory results for participant A2. A case description is provided in Supplementary File 2. Red line indicates the first HIV positive visit; blue line indicates the first site positive visit; bracket shows the number of days between these 2 visits. Green lines indicate CAB injections. The X-axis indicates the number of weeks since the first HIV-positive test result, the left Y-axis indicates viral load, and the right Y axis indicates CAB concentration. Horizontal lines indicate CAB cutoffs (1.33 µg/mL = 8 × PA-IC₉₀; 0.664 µg/mL = 4 × PA-IC₉₀; 0.166 µg/mL = 1 × A-IC₉₀; BLQ <0.025 µg/mL). A target concentration >8 × PA-IC₉₀ was used to interpret drug concentrations. Results from retrospective testing performed at the HIV Prevention Trials Network Laboratory Center are shown above the graph: + reactive or positive test result, − nonreactive or negative test result. Viral load values are shown (HIV RNA copies/mL). HIV genotyping results are shown at the top; results from the low viral load single-genome integrase sequencing (low VL SGS-IN) assay are highlighted. All INSTI RAMs are shown; major INSTI RAMs are bolded. Abbreviations: Ag/Ab, antigen/antibody test; BLQ, below limit of quantification; CAB, cabotegravir; HIV, human immunodeficiency virus; INSTI, integrase strand transfer inhibitor; PA-IC₉₀, in vitro protein-adjusted 90% CAB inhibitory concentration; WT, wild type.