FIG. 4.

Infection of DMVECs with viral supernatants of PEL cell lines: immunohistochemical staining of LANA in primary DMVECs following viral infection. Viral supernatants from JSC-1, BC-3, or BCP-1 (data not shown) cells were used to infect DMVECs that were seeded to dual-well culture slides. At 4, 9, 12, 15, and 22 days postinfection (P.I.), cells were fixed and analyzed for LANA expression. DMVECs infected with JSC-1 supernatant showed nuclear LANA staining at day 4. Note that the LANA-positive cells were still round at day 4. By day 9 postinfection, more cells expressed LANA, and these were spindle shaped. Supernatant from BC-3 and BCP-1 cell lines yielded only weak LANA expression and then were detected only at 22 days postinfection. Magnification, ×100.