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. 2023 Apr 12;31(6):874–888. doi: 10.1016/j.jsps.2023.04.006

Table 2.

Toxicity studies of different plant parts from P. macrocarpa.

No Plant part Plant extract Type of toxicity study Animal and Dose Duration Observation Reference
1 Ripe fruits (Mesocarp & pericarp) Ethanol Acute toxicity Brine shrimp naupli (Artemia salina)
0.78 ug/ml − 800 ug/ml
24 h Safe and non-toxic. LC50 > 800 ug/ml (Azad, 2016)
2 Fruits Ethanol Acute toxicity Adult SD rats
2000, 5000 mg/kg BW
14 days No mortality, no hepatoxic and no nephrotoxic (Shwter et al., 2016)
3 Fruits (Mesocarp & pericarp) Ethanol Acute toxicity Healthy male SD rats
5000 mg/kg BW
7 days No mortality, no sign of toxicity; physical behavior, central nervous system, and autonomic nervous system working well (Azad and Sulaiman, 2020)
4 Fruits Petroleum ether Acute toxicity Healthy adult female SD rats
2000 mg/kg BW
14 days No sign of toxicity and no changes in animal behavioral pattern. (Altaf et al., 2018)
Chloroform No sign of toxicity and no changes in animal behavioral pattern.
Methanol No sign of toxicity and no changes in animal behavioral pattern.
Water No sign of toxicity and no changes in animal behavioral pattern.
5 Fruits (Mesocarp & pericarp) 70% ethanol Acute toxicity Healthy adult male SD rats
500, 1000, 2000, 5000 mg/kg BW
28 days No body weight changes, no mortality, no diseases, and no significant changes in animal behavior. However, at dose 5000 mg/kg, serum creatinine and urea levels are reduced compared to normal control (Azad et al., 2021)
6 Leaves Ethanol Subchronic toxicity Male and female Wistar rats
100, 500, 1000 mg/kg BW
90 days No toxic effect, AST and ALT level did not increase. No effect in blood hematology. However, liver tissue necrosis observed at dose 1000 mg/kg BW, but it was reversible. (Hanif, 2021)
7 Leaves Ethanol Teratogenic toxicity Female rats (Day 6 to day 15 pregnancy)
100, 500, 1000 mg/kg BW
19 days No abnormalities, however, 3 fetuses with incomplete sternum at dose 1000 mg/kg BW were observed. (Hanif, 2021)
8 Unknown part Methanol Teratogenic toxicity Zebra fish embryos
10, 100, 1000 ug/ml
96-hour post fertilisation No teratogenic effects (Omar et al., 2020)

AST-Aspartate aminotransferase; ALT-Alanine transaminase; BW-Body weight; LC50-Lethal concentration at 50%; SD- Sprague Dawley.