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. 2023 Apr 3;128(11):2013–2024. doi: 10.1038/s41416-023-02253-7

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Fig. 4 — CDDP-resistant cells demonstrated increased fluorescence lifetime of NAD(P)H at an individual cell level (scale bar = 60 μm) (a) which is due to increased lifetimes of both free and protein-bound (or adjacent) NAD(P)H with a concomitant reduction in the REDOX ratio of FAD/(FAD + NAD(P)H) (b). Biochemical measurements confirm a significant increase in the ratio of NADH/NAD + in all three cell lineages under normal growth conditions. Total glutathione (GS) measured biochemically and normalised to cell number (d) is significantly higher in CDDP-resistant clones as is reduced glutathione (GSH) measured using fluorescence (e). (*P < 0.05; **P < 0.01; ***P < 0.001). Data for (c–e) are presented as means + /− standard error of the mean.