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. 2023 Apr 3;128(11):2013–2024. doi: 10.1038/s41416-023-02253-7

Fig. 5. CDDP-resistant cells have reduced energy production.

Fig. 5

a Biochemical measurements of lactate dehydrogenase (LDH) activity demonstrated a 20–50% reduction in activity in CDDP-resistant clones compared to parental cell lines (data presented as means with +/− standard error of the mean). b No alterations in mitochondrial integrity, morphology or number were detected in CDDP-resistant clones. c Representative fluorescence images of cells stained with MitoTracker and DAPI, with quantified mitochondria number per nucleus. Data are represented as the mean ± SEM (n > =3, at least 50 cells per experiment). Scale bar is 10 μm. Red fluorescence, mitochondria; blue fluorescence, DAPI-labelled nucleus. d Despite this HN30R4E1 and HN30R4F5 both demonstrated a ~50% reduction in spare respiratory capacity and oxidative phosphorylation-dependent ATP production compared to HN30 (measured using Seahorse; OCR = oxygen consumption rate; data normalised to cell number; data presented as means with +/− standard deviation). (*P < 0.05; **P < 0.01; ***P < 0.001).