Table 2.
Characterization vectors for each processed BC cell line
| Cell Line | Previously Published subtype | Cell subtype | Tumor | Location | ER | PR | HER2 | BRCA1 | Heterogeneity | Stemness | High-resolution subtyping | Marker Identification |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SKBr3 | HER2 + | Epithelial | AC | Metastasis | - | - | + | - | 2 | 7 | HER2 + | CTAG2 |
| MDA-MB-361 | HER2 + / Luminal B | Epithelial | AC | Metastasis | + | ± | + | - | 4 | 2 | TNBC | SRGN |
| BT-474 | HER2 + / Luminal B | Epithelial | Invasive DC | Primary | + | + | + | - | 3 | 9 | HER2 + | FAM3B |
| MDA-MB-453 | HER2 + / TNBC / Unclassified | Epithelial | AC | Metastasis | - | - | + | - | 3 | 10 | HER2 + & TNBC | ANKRD30A |
| MCF-7 | Luminal A | Epithelial | Invasive DC | Metastasis | + | + | - | - | 4 | 4 | Luminal | TFF1 |
| T47D | Luminal A | Epithelial | Invasive DC | Metastasis | + | + | - | - | 5 | 6 | Luminal | SERPINA6 |
| MDA-MB-468 | TNBC | Epithelial | AC | Metastasis | - | - | - | - | 3 | 12 | TNBC | SLC1A6 |
| SUM190 | TNBC / HER2 + | Epithelial | Inflammatory DC | Primary | - | - | + | - | 3 | 11 | HER2 + & TNBC | ALDH3A1 |
| HCC1954 | TNBC / HER2 + | Epithelial | DC | Primary | - | - | + | - | 3 | 13 | HER2 + | FAM9C |
| SUM149 | TNBC | Epithelial / Mesenchymal | Inflammatory DC | Primary | - | - | - | + | 2 | 8 | TNBC | WFDC2 |
| BT-549 | TNBC / Claudin-Low | Mesenchymal | Invasive DC | Primary | - | - | - | - | 5 | 5 | TNBC | COL1A2 |
| MDA-MB-436 | TNBC / Unclassified | Mesenchymal | AC | Metastasis | - | - | - | + | 4 | 3 | TNBC | MAGEA4 |
| MDA-MB-231 | TNBC / Unclassified / Claudin-Low | Epithelial / Mesenchymal | AC | Metastasis | - | - | - | - | 2 | 1 | TNBC | SRGN |
This table is a summary of current publicly available understandings of each cell line as well as novel identifiers and characterization vectors from the analyses discussed here. Heterogeneity is a ranking system based on cluster counts generated from a standard resolution parameter. Stemness is the ranking score based on custom entropy scoring predictions. Higher-resolution subtyping clarifies some of the ambiguating in cell line disease subtyping. Marker identification is a significant and isolated representative gene in each cell line compared to the remaining global dataset