Table 1.
Comparison between p53 expression of patients and controls
| Cases (n = 25) | Control (n = 15) | ||
|---|---|---|---|
| Before | After | ||
| P53 protein concentration by ELISA (concentration of P53/ mg protein) | |||
| Median (Min.–Max.) | 94.8 (49.9–121) | 183.3 (129.3–503) | 161 (114–202) |
| Mean ± SD | 90.78 ± 23.58 | 225.9 ± 105.3 | 160.93 ± 31.03 |
| Significance | Zp1 < 0.001*, Up2 < 0.001*, Up3 = 0.052 | ||
| P53 gene expression by RT-PCR | |||
| Median (Min.–Max.) | 0.04 (0.0–0.20) | 0.71 (0.48–0.98) | 0.27 (0.20–0.31) |
| Mean ± SD | 0.07 ± 0.06 | 0.72 ± 0.17 | 0.26 ± 0.04 |
| Significance | Zp1 < 0.001*, Up2 < 0.001*, Up3 < 0.001* | ||
| Immunohistochemical expression of P53 | |||
| Grade 0 | 19 (76.0%) | 0 (0.0%) | 10 (66.7%) |
| Grade + 1 | 4 (16.0%) | 5 (20.0%) | 2 (13.3%) |
| Grade + 2 | 2 (8.0%) | 8 (32.0%) | 3 (20.0%) |
| Grade + 3 | 0 (0.0%) | 12 (48.0%) | 0 (0.0%) |
| Significance | MHp1 < 0.001*, MCp2 = 0.521*, MCp3 < 0.001* | ||
p1: p value for comparing between before and after; p2: p value for comparing between cases (before) and control; p3: p value for comparing between cases (after) and control
SD standard deviation, U Mann–Whitney test, Z Wilcoxon signed ranks test, MH Marginal homogeneity test, MC Monte Carlo (χ2 test)
*Statistically significant at p ≤ 0.05