Figure 4.

Antibody responses in chronic herpes simplex virus (HSV) 2 infection in human immunodeficiency virus (HIV)–uninfected (HIV⁻) and HIV-infected (HIV⁺) women. A, Samples from HIV⁻ and HIV⁺ women were assayed for total IgG. B, HSV-specific immunoglobulin (Ig) G, IgG1, IgG3, and C1q-binding antibodies assayed by enzyme-linked immunosorbent assay. Abbreviation: OD₄₅₀, optical density at 450 nm C, Neutralization titers assayed by plaque reduction assay. Abbreviation: IC₅₀, serum dilution that inhibited 50% of infection. D, Antibody-dependent cell-mediated cytotoxicity (ADCC) assessed using a Promega human FcγRIIIa (Fc gamma receptor IIIa) ADCC Reporter Bioassay and expressed as fold induction. Open symbols represent women who reported HSV-2 recurrences within the previous 12 months. *P < 0.5; **P < 0.01 (Mann-Whitney U test).