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. 2023 May 23;13(5):e1261. doi: 10.1002/ctm2.1261

FIGURE 3.

FIGURE 3

C1QTNF4 reduces wire injury–induced neointima formation in transgenic mouse carotid arteries. (A, B) Representative HE staining of 8‐week‐old C1QTNF4 transgenic (C1QTNF4‐TG) and sex‐matched wild‐type (WT) mouse common carotid arteries on the 0th, 14th and 28th days after wire injury. Scale bar, 50 μm. (C, D) Quantitative analysis of the neointimal area on the 14th and 28th days (C), and the neointima/media ratio on the 14th and 28th days (D) (± SEM; n = 6 per group). (E) Representative immunofluorescent staining of WT and C1QTNF4‐TG mouse common carotid arteries at 14 days after wire injury. Slides were simultaneously stained with a multiplex QIF panel containing C1QTNF4 (cyan), α‐SMA (red), Ki67 (yellow), EL (green) and DAPI (blue). Scale bar, 30 μm. (F) Quantification of the expression of C1QTNF4 and Ki67 positive cells in the injured arteries (± SEM, n = 9). ****p < .0001.