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. 2023 May 10;17:1176587. doi: 10.3389/fnins.2023.1176587

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Copyright © 2023 Ros-Bernal, Gil-Miravet, Lucerón, Navarro-Sánchez, Castillo-Gómez, Gundlach and Olucha-Bordonau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RXFP3 expression and RLN3 distribution in the septal area of postnatal rats (A). RT-PCR analysis of RXFP3 mRNA levels in the septal area of P3, P10, P20 and 9 month-old rats revealed a peak at P10 and a normalization to adult concentrations by P20. Data are presented as mean ± SEM and were analyzed using one-way ANOVA followed by the LSD-text (*p < 0.05, **p < 0.01, ***p < 0.005) (B–D). Immunohistochemistry for RLN3 revealed a few fibers at P3 (B), dispersed RLN3 labeling in the septal area of P10 rats (C), and dense RLN3 fibers in the horizontal diagonal band at P20 (D). In (C), the arrow highlights a RLN3 fiber. Calibration bar, 50 μm.