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. 2023 May 10;17:1176587. doi: 10.3389/fnins.2023.1176587

Figure 2.

Figure 2

Development of the RLN3 innervation of the MS0 (A). Violin plots of RLN3 fiber density during postnatal development from P10 to P20 and in adult rats (B–L). Maximal projections of a total stack of 5 μm images displaying double-labeling of calcium-binding proteins or ChAT (red) and RLN3 (green) (B,C). Double-labeling at P3 for ChAT and RLN3 (B), and PV and RLN3 (C). (D–F) double-labeling at P10 for CB28kD + RLN3 (D), PV + RLN3 (E) and CR + RLN3 (F). (G–I) double-labeling at P15 for CB28kD + RLN3 (G), PV + RLN3 (H) and CR + RLN3 (I). (J–L) double-labeling at P17 for CB28kD + RLN3 (J), PV + RLN3 (K) and CR + RLN3 (L). An increased density in RLN3 fibers occurred between P15 (G–I) and P17 (J–L). Arrows point at RLN3 fibers, arrowheads point at putative contacts because of a close apposition between a positive RLN3 fiber and an IF structure. Calibration bar (B–L), 50 μm. Colored symbols in bars represent trends and statistically significant differences among groups after post hoc analysis: *p < 0.05, **p < 0.01, ***p < 0.001.