Table 2.
Primer sequences used for qPCR gene expression analysis.
| Target | Forward primers | Reverse primers | Genbank ID |
|---|---|---|---|
| genes | (5′ to 3′) | (5′ to 3′) | |
| IGF-1 | ATCTCTTCTAC CTGG | GAAGCAACACTCAT | AH002176.2 |
| CACTCTG | CCACAAT | ||
| VEGF | GCACCCACG AC | TGAACGCTC CA | AY033504.1 |
| AGAAGG | GGATTTA | ||
| TGF-β1 | CTGAACCAAGG AGACG | GTTTGGGACT GATCC | NM_021578.2 |
| GAATAC | CATTGA | ||
| EGF | ACCAACACGGA GGGA | GCGGTCCACGGA | NM_012842.1 |
| GGCTACAA | TTCAACATACA | ||
| MMP-9 | ATCTCTTCTAGAGACT | CAAGCTGATT GGTT | NM_031055 |
| AGGAAGGAG | CGAGTAGC |
IGF-1, insulin-like growth factor-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; EGF, endothelial growth factor; MMP-9, matrix metalloproteinase-9.