Figure 5.

The MITF inhibitor ML329, reduces degranulation and Ca2⁺ influx. (A) LAD2 cells were treated with either DMSO or ML329 at different concentrations on various days, and the efficiency of MITF silencing was assessed by western blot. α-Tubulin was used as a loading control. The unpaired t-test was used for statistical analysis after 5 days (*p < 0.05, ****p<0.0001). Experiments are the mean ± SEM (n=4). (B) Degranulation was determined by flow cytometry using CD63 staining in unstimulated and ML329 -treated cells (after 5 days) in the live population (propidium iodide negative staining). (C) DMSO and ML329 -treated cells (after 5 days) were preincubated with Fluo4 and activated with SP (2µM) or ionomycin. A two-way ANOVA test was used for statistical analysis (***p<0.001, ****p<0.0001). Experiments are the mean ± SEM (n=3).