Figure 7.

Drug activation of MRGPRX2 induces MITF activity. Cells were activated with SP or drugs (vancomycin, atracurium, morphine, and meglumine amidotrizoate) reported to induce MRGPRX2-dependent MC degranulation at various concentrations for 6 hours in LAD2 cells transfected with TRPM1-luciferase gene reporter. Experiments are the mean ± SEM (n=3 for vancomycin and atracurium, n=4 for meglumine amidotrixoate and morphine). The unpaired t-test was used for statistical analysis (*p < 0.05, **p < 0.01, ****p < 0.0001).