Fig. 7.

The SIRT1 inhibitor, EX-527, reversed the protective effects of NMN against apoptosis and microglial activation. (A, B) Representative immunofluorescent staining images and quantification of TUNEL (green) with DAPI (blue) in the DG region of hippocampus. Scale bar = 20 μm. (C) Representative immunofluorescent staining images of Iba1+ (green), CD68⁺ (red) with DAPI (blue) in the DG region of hippocampus. Scale bar = 20 μm. (D, E) Statistical diagram of the number of Iba1+ microglia cells and the ratio of CD68⁺ Iba1+ cells/Iba1+ cells in the DG region of hippocampus. Tukey post-hoc test following one-way ANOVA was used. The values were presented as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, n = 3 or 6 for each group).